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Gel Electrophoresis

Agarose Gel Analysis

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Learn how to visualise and interpret DNA bands in an agarose gel using a transilluminator. Practise identifying the number of bands in a sample, labelling the bands of a DNA ladder and estimating the size of sample DNA fragments.

Learning Outcomes

  • Use a transilluminator to visualise the bands on an agarose gel
  • Identify samples according to the number of different sized fragments of DNA they contain
  • Label the DNA ladder, and use this to estimate the size of some of the bands
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Interpreting and Analysing Data

  • How a transilluminator helps visualise the results of an agarose gel
  • How to identify the number of different sized DNA fragments in a sample
  • Why a DNA ladder is loaded alongside the samples
  • How the different sized DNA ladder bands migrate in relation to each other
  • How to use the DNA ladder bands to estimate the size of sample DNA fragments

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Prof. Mike Davies-Coleman
University of the Western Cape

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Gel Electrophoresis

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