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Gel Electrophoresis

Agarose Gel: Separating DNA by Size

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Learn how DNA moves through an agarose gel placed in a conductive buffer when a current is applied. Practise predicting the relative migration speeds of different-sized DNA fragments.

Learning Outcomes

  • Examine the movement of DNA through a conductive solution
  • Learn how an agarose gel placed in conductive buffer is able to separate DNA by size
  • Predict the relative migration speeds of various lengths of DNA travelling through an agarose gel
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Concepts Principles and Theories

  • How the application of an electrical current affects the movement of DNA through the gel
  • Why the movement of DNA molecules through the gel is affected by their size
  • How DNA molecules of differing sizes move in relation to each other

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Dr Sheila Faherty
Altantic Technological University

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Gel Electrophoresis

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