Immunodetection

Analysing ELISA

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Explore all the analysis steps needed to interpret novel ELISA data. Recall ELISA principles, then define the dilution series and build a standard curve. Use that standard curve to interpret the concentration of protein from an ELISA experiment. Then, put these skills into context by applying them to a novel scenario to reach an evidence-based conclusion.

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Learning outcomes

Recall the principles of ELISA and apply relevant calculations to absorbance data.
Calculate different ways of diluting protein and construct graphs to interpret those dilutions.
Calculate the coefficient of variation and use it to assess reliability.
Rearrange and use the equation of a line to solve protein concentration.
Evaluate data to reach an evidence-based conclusion.

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Skills

Manipulating and Presenting Data

How to calculate the volume needed to resuspend a lyophilised protein
How to calculate how much stock solution would be required for a specific dilution
How to calculate a dilution factor
How to calculate a serial dilution
How to calculate mean corrected absorbance
How to calculate the coefficient of variation
How to rearrange the equation of the standard curve to solve for protein concentration
How to plot a standard curve and determine an evidence-based outcome using the previous learned skills

Interpreting and Analysing Data

Determining if protein measurements are reliable based on the calculated coefficient of variation
Reaching an evidence-based conclusion using gathered data calculated from a novel scenario

Concepts, Principles and Theories

Identifying the fundamental principles of ELISA and the reasons behind certain practices for the method and analysis

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Dr. Alexandra Moores

School of Biosciences, University of Kent

The Smart Worksheet was extremely useful to see the overall understanding and application of theories that we expected students to be able to use. The feedback is clear and accessible, enabling students to gain a deeper understanding of the topic they are studying and aiding in their success.

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Degrees

Biological science

Biomedical science

Pharmaceutical science

Modules

Molecular biology

Topic

Immunodetection

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