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Clinical immunology

Identification, characterisation and diagnosis: monoclonal gammopathies

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Navigate the complex procedures involved in the diagnosis of monoclonal gammopathies. Interpret differences between normal and abnormal serum protein electropherogram and immunofixation outputs. Study patterns of haematological, clinical biochemistry and protein-level data in order to formulate a diagnostic conclusion.

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Learning outcomes

  • Apply knowledge of molecular immunology and the laboratory diagnostic techniques relevant to clinical immunology.
  • Interpret normal and abnormal serum protein electropherograms.
  • Make assessments on the spontaneity of the reaction based on the sign of the Gibbs free energy change.
  • Interpret immunofixation patterns to identify isotypes of monoclonal immunoglobulins.
  • Analyse and interpret haematological and clinical biochemistry data relevant to monoclonal gammopathies..
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Skills

Manipulating and Presenting Data

  • Calculate the fraction concentration for electropherogram data

Interpreting and Analysing Data

  • Interpret electropherogram and immunofixation data and evaluate those outputs in a clinical context to reach relevant diagnostic and prognostic conclusions

Concepts, Principles and Theories

  • Explore the background of the anatomy and interpretation of capillary zone electrophoresis, the output electropherograms, immune structure and function, and how they interconnect in a clinical context

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Dr Alan Courtney, Senior Biochemist, Imperial Healthcare NHS

“This particular worksheet should prepare scientists for interpretation of the commonly used laboratory techniques for diagnosis and monitoring of monoclonal gammopathies. It involves the use of a variety of skills, particularly pattern recognition, but also linking up with the common limitations of the methods, the clinical background and other clinical and laboratory data.”

Details

Subject areas

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Discipline

Clinical biomedical science

Topic

Clinical immunology

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