Agarose gel electrophoresis

Loading and running an agarose gel

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Learn the key steps in loading and running an agarose gel. Practise pouring a buffer solution, removing the comb, loading a DNA sample and setting up the power pack.

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Learning outcomes

Familiarise yourself with the equipment needed for agarose gel electrophoresis.
Prepare a gel for loading.
Load samples into a gel.
Set up a power pack to run a gel.

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Skills

Using Instruments and Equipment

What equipment is required to run an agarose gel
What components of the buffer facilitate the loading, tracking and visualisation of samples
How to position the pipette to load the samples into the wells
Why it is important to correctly wire the electrodes to the tank
Why it is best to remove the comb after pouring the buffer

Obtaining and Recording Data

How the amount of buffer added affects the running of the gel
How the voltage selected can affect the running of the gel and the quality of the results
Why a DNA ladder is loaded alongside the samples

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Student, Natural Sciences

University of Exeter

“I really like labs but it can be challenging because the apparatus is new and very different to school. I use LabSims during the week on my laptop and make notes the night before a lab. Seeing it in the simulation then the lab made it more familiar and helped me feel like I knew what I was doing. I could make mistakes in the simulation and learn from that first.”

Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Agarose gel electrophoresis

Purchase option

Bioscience LabSim Library

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