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Solutions & Dilutions

Preparing Serial Dilutions

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Learn and practise the key steps necessary to perform a 10-fold serial dilution. Consider the changes in concentration and assign the correct final concentration to each tube, and select the appropriate pipette and settings to transfer and mix the stock and diluent solutions.

Learning Outcomes

  • Perform a 10-fold serial dilution with step-by-step guidance
  • Practise serial dilutions more independently
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Designing and Planning Experiments

  • How to select the appropriately sized pipette for transferring the stock and diluent

Using Instruments and Equipment

  • How to set the volume on the pipette display to transfer the correct amount of solution
  • How to mix the solution thoroughly after combining

Concepts Principles and Theories

  • What is meant by the terms stock, diluent and serial dilution
  • How to identify the concentration and volumes required for each step in a 10-fold dilution

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