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Spectroscopy & Spectrometry

Calibration Curves in Spectrophotometry

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Learn about calibration curves and their applications. Practise generating a calibration curve and estimating the protein concentration of some unknown samples from their absorbances.

Learning Outcomes

  • Consider when it may be appropriate to use a calibration curve
  • Generate a calibration curve using different concentrations of a stock solution
  • Use a calibration curve to determine the concentration of an unknown solution
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Designing and Planning Experiments

  • When is it appropriate to use a calibration curve vs the Beer-Lambert Law
  • What is required to generate a calibration curve 
  • How to choose a sensible range of protein concentrations to generate a calibration curve for a Bradford assay

Interpreting and Analysing Data

  • How to use a calibration curve to estimate the protein concentrations of samples

Concepts Principles and Theories

  • How a Bradford assay can be used to assess total protein concentration in a sample

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