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Spectroscopy & Spectrometry

UV-Vis Spectrophotometer

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Practise safely and effectively using a spectrophotometer, including how to fill the cuvette to the correct level, how to place it in the correct orientation, and how to adjust the lid position for accurate absorbance readings.

Learning Outcomes

  • Familiarise yourself with the basic principles of spectrophotometry
  • Understand how to set up and use a spectrophotometer
  • Learn how to fill the cuvette to the appropriate level, place it in the correct orientation and adjust the lid position to obtain an accurate absorbance reading
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Using Instruments and Equipment

  • How much sample should be poured into the cuvette 
  • Why the cuvette must be positioned with the light beams passing through the transparent sides
  • Why the lid of the spectrophotometer should be closed when taking readings 

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School of Chemistry, University of Glasgow

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