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Spectroscopy & Spectrometry

UV-Vis Spectrophotometer

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Practise safely and effectively using a spectrophotometer, including how to fill the cuvette to the correct level, how to place it in the correct orientation, and how to adjust the lid position for accurate absorbance readings.

Learning Outcomes

  • Familiarise yourself with the basic principles of spectrophotometry
  • Understand how to set up and use a spectrophotometer
  • Learn how to fill the cuvette to the appropriate level, place it in the correct orientation and adjust the lid position to obtain an accurate absorbance reading
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Skills

Using Instruments and Equipment

  • How much sample should be poured into the cuvette 
  • Why the cuvette must be positioned with the light beams passing through the transparent sides
  • Why the lid of the spectrophotometer should be closed when taking readings 

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Dr. Ciorsdaidh Watts
School of Chemistry, University of Glasgow

“After using LabSims, 90% of our students felt better prepared for the lab, an increase of 50% from the previous year. We’ve noted how much extra time they had in the lab to concentrate on techniques and safety. Now, we have nearly 2,000 chemistry students using LabSims and Smart Worksheets."

Details

Degrees

Pharmacological Science
Forensic Science
Chemistry

Modules

Analytical Chemistry

Topic

Spectroscopy & Spectrometry

Purchase option

Chemistry LabSim Library

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