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High Performance Liquid Chromatography (HPLC)

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Learn and compare the properties of HPLC column and a standard glass column. Explore how altering column properties such as efficiency, selectivity column length, particle size and relative polarity affects the performance. Review the two modes of HPLC separation and predict the order of elution for different compounds.

Learning Outcomes

  • Familiarise yourself with key components of HPLC
  • Explore the relationship between the column properties and sample resolution
  • Examine how the choice of phases can affect the order of elution
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Designing and Planning Experiments

  • How normal and reverse phase HPLC differ
  • How HPLC compares to standard column chromatography

Using Instruments and Equipment

  • What key components make up the HPLC instrument

Obtaining and Recording Data

  • How column efficiency and selectivity relate to resolution 
  • How column length and particle size affect column efficiency
  • How the polarity of the mobile and stationary phases affect column selectivity

Interpreting and Analysing Data

  • What is meant by resolution 
  • How bands in the column relate to chromatogram peaks

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Dr. Magdalena Wajrak
Edith Cowan University

"I wish I had simulations like these when I was learning chemistry. Chemistry in particular is such a visual subject, a lot of students need that visualisation, so I think they're lucky to have these resources”



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