Chromatography

HPLC analysis

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Apply the theory of separation in HPLC to tackle questions about order of elution and the retention factor. Analyse HPLC chromatograms and practise calculating the mathematical variables which describe separation in HPLC before applying your knowledge to a method development scenario.

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Learning outcomes

predict the order of elution of different analytes based on their polarities.
predict the effect of altering HPLC system parameters on analyte retention and separation quality.
calculate retention factor, selectivity, efficiency and resolution from a chromatogram.

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Skills

Using Instruments and Equipment

How to distinguish between normal-phase and reverse-phase HPLC
Identify different parameters which can be changed during HPLC analysis and predict their effect on retention and separation quality

Interpreting and Analysing Data

How to analyse data from HPLC chromatograms to calculate retention factor, efficiency, selectivity and resolution

Concepts, Principles and Theories

Identify the correct order of elution for compounds of different polarities in normal and reverse-phase HPLC

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Student

University of Glasgow

“It’s just at that point where it is not telling you the answer but it’s helping you get there."

Details

Degrees

Chemistry

Foundation science

Pharmaceutical science

Discipline

Analytical chemistry

Topic

Chromatography

Purchase option

The Smart Worksheet Library

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