Chromatography

HPLC analysis

No items found.

Apply the theory of separation in HPLC to tackle questions about order of elution and the retention factor. Analyse HPLC chromatograms and practise calculating the mathematical variables which describe separation in HPLC before applying your knowledge to a method development scenario.

Request a demo

See skills

arrow_downward

Learning outcomes

Predict the order of elution of different analytes based on their polarities.
Predict the effect of altering HPLC system parameters on analyte retention and separation quality.
Calculate retention factor, selectivity, efficiency and resolution from a chromatogram.

No items found.

chevron_left

chevron_right

Skills

Using Instruments and Equipment

How to distinguish between normal-phase and reverse-phase HPLC
Identify different parameters which can be changed during HPLC analysis and predict their effect on retention and separation quality

Interpreting and Analysing Data

How to analyse data from HPLC chromatograms to calculate retention factor, efficiency, selectivity and resolution

Concepts, Principles and Theories

Identify the correct order of elution for compounds of different polarities in normal and reverse-phase HPLC

Learn more about our approach to skills

Student

University of Glasgow

“It’s just at that point where it is not telling you the answer but it’s helping you get there."

Details

Degrees

Chemistry

Foundation science

Pharmaceutical science

Modules

Analytical chemistry

Topic

Chromatography

Purchase option

The Smart Worksheet Library

Related products

Chromatography

Gas chromatography analysis

Smart Worksheet

Chromatography

Thin layer chromatography (TLC) analysis

Smart Worksheet

Chromatography

High performance liquid chromatography (HPLC)

LabSim

Chromatography

Size exclusion chromatography

LabSim

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.

Enhance student skills and accelerate learning with multiple opportunities to practise
Boost confidence and competency with enhanced support and personalised feedback
Engage and motivate with enjoyable, challenging learning experiences

LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice

Improve the student experience of assessment with immediate, targeted feedback
Save academic time by minimising repetitive marking for staff
Gain immediate insight into student progress with individual performance data

Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.

Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

Try our resources for free
Get a live demonstration of our products
Become a partner and join our community
Talk to our team of experts

Request a demo

Image of a smiling student with a microscope