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Polymerase Chain Reaction (PCR)

Key Stages in a Polymerase Chain Reaction (PCR)

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Learn about the key steps involved in a polymerase chain reaction. Explore each stage of a PCR cycle: denaturation, primer annealing, and primer extension.

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Learning Outcomes

  • Explore, in detail, the stages of one PCR cycle
  • Answer questions on the theory of PCR
  • Consider the power of DNA amplification by doubling
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Skills

Concepts Principles and Theories

  • What happens during one cycle of amplification
  • What reagents are required to carry out PCR
  • How changes in temperature initiate the different phases of PCR
  • How multiple cycles of PCR can be used to amplify a region of DNA

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Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."

Details

Degrees

Biochemistry
Biological Science
Biomedical Science
Forensic Science
Pharmacological Science

Modules

Biological Chemistry
Molecular Biology & Genetics
Forensic Investigation

Topic

Polymerase Chain Reaction (PCR)

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Bioscience LabSim Library

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