Genetics

Running a polymerase chain reaction (PCR)

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Learn how to set up a PCR reaction by preparing four tubes for an experiment, including a positive and negative control. Explore the steps of adding dNTPs, primers, samples and enzymes to each tube, and set the thermocycler to the correct settings. Analyse the results by running samples on an agarose gel and interpreting the outcome.

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Learning outcomes

Add key reagents of a PCR experiment to four PCR tubes and consider their function.
Set up negative and positive controls.
Analyse the results of the PCR by studying an agarose gel of the amplified DNA.

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Skills

Designing and Planning Experiments

Why dNTPs, DNA, primers and polymerase enzyme are required in each sample tube
Where primers should be targeted in relation to the region of interest
What makes an appropriate positive and negative control in a PCR experiment

Using Instruments and Equipment

How to set the desired cycle settings on the thermocycler

Obtaining and Recording Data

Why it is important to prepare PCR samples on ice
Why it is best to add the DNA polymerase to the reaction mix last

Interpreting and Analysing Data

How to use an agarose gel to identify whether samples and controls contain the region of interest

Learn more about our approach to skills

Dr. Sharon Williams

Coventry University

“Before attending the labs, students are encouraged to use the bioscience or chemistry materials to understand the practicals better. This makes the students more confident when attending the labs.”

Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Genetics

Purchase option

Bioscience LabSim Library

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