Genetics

Understanding qPCR

No items found.

Learn about incorporating quantification in PCR. Explore the principles of fluorescence and two different fluorescence detection methods used in qPCR. Understand the progression of a standard amplification plot and review a set of qPCR results.

Request a demo

See skills

arrow_downward

Learning outcomes

Review the fundamentals of PCR and its quantification.
Identify the principles and differences of two fluorescence methods.
Recognise the progression of a standard amplification plot.

No items found.

chevron_left

chevron_right

Skills

Designing and Planning Experiments

How SYBR green and TaqMan probes differ in their specificity for DNA, interaction with DNA and cost
What are some of the key differences between endpoint PCR and qPCR

Interpreting and Analysing Data

What axes are used for an amplification plot
How does the curve on an amplification plot reflect what is happening on a molecular level in the sample
How to use an amplification plot to compare the starting quantities of target DNA

Concepts, Principles and Theories

What are the 3 main stages of a PCR cycle and the temperatures at which they occur
What is meant by the term fluorophore
Why an increase in target DNA proportionally increases the fluorescence by SYBR green
Why an increase in target DNA proportionally increases the fluorescence by TaqMan probes
Why the process is called qPCR or RT-PCR

Learn more about our approach to skills

Student response from Students Insights Survey

“I like that it goes through all the steps, so you have a better understanding of what you have to do for the actual lab... It makes me feel more prepared.”

Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Genetics

Purchase option

Bioscience LabSim Library

Related products

Genetics

qPCR in molecular diagnostics

Smart Worksheet

Genetics

PCR primer design

Smart Worksheet

Genetics

Running a polymerase chain reaction (PCR)

LabSim

Genetics

Key stages in polymerase chain reaction (PCR)

LabSim

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.

Enhance student skills and accelerate learning with multiple opportunities to practise
Boost confidence and competency with enhanced support and personalised feedback
Engage and motivate with enjoyable, challenging learning experiences

LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice

Improve the student experience of assessment with immediate, targeted feedback
Save academic time by minimising repetitive marking for staff
Gain immediate insight into student progress with individual performance data

Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.

Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

Try our resources for free
Get a live demonstration of our products
Become a partner and join our community
Talk to our team of experts

Request a demo

Image of a smiling student with a microscope