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Operating an Automated Pipette

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Learn the steps involved in operating an automated pipette correctly to transfer liquid between vessels. Practise transfering specific volumes of solution between vessels.

Learning Outcomes

  • Familiarise yourself with the controls on a pipette and their functionality
  • Draw a specified volume of solution from a beaker into the disposable tip
  • Dispense the solution into an empty microfuge tube
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Using Instruments and Equipment

  • What are the key controls on a micropipette
  • How to correctly position the pipette to aspirate and dispense liquid 
  • How to depress and release the plunger to the correct stops to withdraw and dispense liquid
  • Why the pipette should not be turned on its side when there is liquid in the tip 

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University of the Western Cape

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