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Light Microscopy

Setting up a Microscope

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Learn the key components of a microscope. Explore adjusting the brightness, interocular distance, stage height and objective lens to view a sample.

Learning Outcomes

  • Familiarise yourself with the key components of a microscope and their individual functions
  • Select the optimal settings to begin viewing a specimen using a microscope
  • Work through the steps involved in configuring a microscope for Köhler illumination
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Using Instruments and Equipment

  • What are the main components of a compound light microscope 
  • How to set the brightness to a suitable level 
  • How to adjust the eyepiece to match the interpupillary distance
  • Why it is best to use the lowest power objective when first examining a slide 
  • How best to position the stage to begin focussing on the sample

Working in a Safe Manner

  • Why care must be taken when raising the stage

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Dr Anna Maria Piccinini
University of Nottingham

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Light Microscopy

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