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Light microscopy

Setting up a Microscope

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Learn the key components of a microscope. Explore adjusting the brightness, interocular distance, stage height and objective lens to view a sample.

Learning outcomes

  • Familiarise yourself with the key components of a microscope and their individual functions
  • Select the optimal settings to begin viewing a specimen using a microscope
  • Work through the steps involved in configuring a microscope for Köhler illumination
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Skills

Using Instruments and Equipment

  • What are the main components of a compound light microscope 
  • How to set the brightness to a suitable level 
  • How to adjust the eyepiece to match the interpupillary distance
  • Why it is best to use the lowest power objective when first examining a slide 
  • How best to position the stage to begin focussing on the sample

Working in a Safe Manner

  • Why care must be taken when raising the stage

Learn more about our approach to skills
Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"

Details

Degrees

Biochemistry
Biomedical science
Forensic science
Pharmacological science
Biological science

Modules

Anatomy & physiology
Tissue & cell biology
Microbiology
Ecology & evolution
Forensic investigation
Immunology

Topic

Light microscopy

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