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Light microscopy

Kohler Illumination

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Learn about Köhler illumination and the benefits of using this technique. Familiarise yourself with the components of a microscope and how to adjust the light source for optimal imaging. Practise adjusting the diaphragm and condenser to achieve optimal contrast and resolution.

Learning outcomes

  • Learn why it is important to set a microscope up for Köhler illumination
  • Familiarise yourself with the parts of a microscope that need to be adjusted to establish Köhler illumination
  • Work through the steps involved in configuring a microscope for Köhler illumination
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Skills

Using Instruments and Equipment

  • What are the steps involved in setting up a microscope for Köhler illumination
  • What is happening mechanically when the field diaphragm is adjusted 
  • How does adjusting the field diaphragm affect the image seen down the microscope
  • What is happening mechanically when the condenser height is adjusted 
  • How does adjusting the height of the condenser affect the image seen down the microscope
  • What is happening mechanically when the condenser centering screws are adjusted 
  • How does adjusting the centering screws on the condenser affect the image seen down the microscope 
  • What is happening mechanically when the iris diaphragm is adjusted 
  • How does adjusting the iris diaphragm affect the image seen down the microscope

Concepts, Principles and Theories

  • What it means to set a microscope up for Köhler illumination

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Details

Degrees

Biochemistry
Biomedical science
Biological science
Pharmacological science
Forensic science

Modules

Anatomy & physiology
Tissue & cell biology
Microbiology
Ecology & evolution
Forensic investigation
Immunology

Topic

Light microscopy

Purchase option

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