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Light Microscopy

Focusing and Navigating

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Learn how to use microscope focus controls to bring an image into focus at 3 different magnifications, and make appropriate adjustments to the brightness with each different objective lens. Practise using the stage controls to bring an area of interest into the centre.

Learning Outcomes

  • Use the focus controls to bring an image into sharp focus at 3 different magnifications
  • Explore the concept of depth of focus and how this changes with increasing magnification
  • Familiarise yourself with the controls used to move the microscope stage and use these to centre the field of view on a region of interest
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Using Instruments and Equipment

  • How to adjust the coarse and fine focus controls to bring an image into sharp focus
  • Why the magnification should be increased incrementally 
  • Why some objective lens’ require the use of immersion oil 
  • How to apply immersion oil 
  • How to adjust the brightness to increase image clarity 
  • How to use the stage controls to bring an area of interest into the centre of the field of view

Obtaining and Recording Data

  • Why care must be taken when raising the stage 
  • Why the coarse focus control should only be used on low power objectives 

Concepts Principles and Theories

  • How the level of magnification affects the depth of focus 
  • How the orientation of the image seen down the microscope compares to the orientation of the sample on the slide

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Dr Sheila Faherty
Altantic Technological University

"The simulations are brilliant reinforcing material, delivered at a good pitch with scope for elaboration and so clear and informative. Students could attempt them many times, and this really helped weaker students build their confidence."



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Light Microscopy

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