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Spectroscopy & Spectrometry

Spectrophotometer: Calibration and Use

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Practise calibrating a spectrophotometer and taking an absorbance reading. Understand the importance of factors such as wavelength, solution type, volume, and cuvette position.

Learning Outcomes

  • Select the appropriate settings to calibrate a spectrophotometer
  • Use a calibrated spectrophotometer to record an absorbance value
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Using Instruments and Equipment

  • How much solution should be poured into the cuvette 
  • Why the cuvette must be positioned with the light beams passing through the transparent sides
  • Why the lid of the spectrophotometer should be closed when taking readings

Obtaining and Recording Data

  • Why diluent should be used as a reference solution to calibrate the spectrophotometer

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