< Back to the full list

Spectroscopy & Spectrometry

Spectrophotometer: Parts and Theory

No items found.

Learn about the parts of a spectrophotometer and the Beer-Lambert Law. Explore the relationship between concentration and absorbance, and the relationship between wavelength.

Request a demo
See skills
arrow_downward

Learning Outcomes

  • Identify key external and internal parts of a spectrophotometer
  • Explore the effect of sample concentration on initial intensity, final intensity and absorbance value - all in relation to the Beer-Lambert law
  • Select an appropriate test wavelength for given absorbance spectra
No items found.
chevron_left
chevron_right

Skills

Designing and Planning Experiments

  • How to select an appropriate wavelength to measure the absorbance of a particular substance

Using Instruments and Equipment

  • What are the main components of a spectrophotometer 
  • What is the function of the three key internal components, the light source, the filter and the detector

Interpreting and Analysing Data

  • What is described by an absorbance spectra

Concepts Principles and Theories

  • How absorbance is calculated 
  • What the relationship is between concentration and initial intensity of light, final intensity and absorbance
  • How the different light and sample variables relate to the Beer-Lambert equation

Learn more about our approach to skills
Student response from our Student Insights pilot survey

“I find using LabSims really helpful as preparation before a practical. It's useful to be able to carry out some of the practical techniques online before completing them in the lab, and they make me feel more confident about attempting these techniques during our lab sessions.”

Details

Degrees

Biochemistry
Biological Science
Biomedical Science
Forensic Science
Pharmacological Science

Modules

Biological Chemistry
Microbiology
Ecology & Evolution
Pharmacology

Topic

Spectroscopy & Spectrometry

Purchase option

Bioscience LabSim Library

Related products

Spectroscopy & Spectrometry

IR: Recording Spectra

LabSim

Spectroscopy & Spectrometry

IR: Fundamentals

LabSim

Spectroscopy & Spectrometry

UV-Vis Spectrophotometer

LabSim

Spectroscopy & Spectrometry

IR: Assigning Peaks

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope