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Thin Layer Chromatography (TLC) Rf Measurement

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Practise taking accurate measurements from a TLC plate to find the distances travelled by the component and the solvent front. Use these to calculate the Rf value.

Learning Outcomes

  • Develop the ability to take accurate distance measurements from TLC plate
  • Understand the concept of Rf values
  • Learn how to calculate Rf values for a component
  • Practise the process of measuring and calculating Rf values for different components
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Obtaining and Recording Data

  • How to measure the distance to the solvent front 
  • How to measure the distance to the spots 
  • How to calculate the Rf value from the distance to the solvent front and spots

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Dr. Magdalena Wajrak
Edith Cowan University

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