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Thin Layer Chromatography (TLC) Setup

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Learn and familiarise yourself with a standard TLC setup. Explore the consequences of adjusting starting spot size, base spot separation and solvent volume, and practise obtaining a clear, well separated chromatogram.

Learning outcomes

  • Familiarise yourself with a standard TLC setup
  • Adjust parameters such as base spot size, base spot separation and solvent volume
  • Understand the consequences of choices made on the clarity of the TLC
  • Learn how to obtain a clear, well separated chromatogram
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Using Instruments and Equipment

  • Where the solvent level should sit in relation to the sample spots
  • Why you should draw the line using pencil rather than pen 
  • Why it is important to cover the container 
  • How you should handle a TLC plate

Obtaining and Recording Data

  • How the size of the spots and the space between them can affect the clarity of the results
  • Why you shouldn’t let the solvent front reach the top of the plate

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Dr. Aga Kosinska
CLIE, University College London

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