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Chromatography

Thin Layer Chromatography (TLC) Setup

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Learn and familiarise yourself with a standard TLC setup. Explore the consequences of adjusting starting spot size, base spot separation and solvent volume, and practise obtaining a clear, well separated chromatogram.

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Learning outcomes

  • Familiarise yourself with a standard TLC setup
  • Adjust parameters such as base spot size, base spot separation and solvent volume
  • Understand the consequences of choices made on the clarity of the TLC
  • Learn how to obtain a clear, well separated chromatogram
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Skills

Using Instruments and Equipment

  • Where the solvent level should sit in relation to the sample spots
  • Why you should draw the line using pencil rather than pen 
  • Why it is important to cover the container 
  • How you should handle a TLC plate

Obtaining and Recording Data

  • How the size of the spots and the space between them can affect the clarity of the results
  • Why you shouldn’t let the solvent front reach the top of the plate

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Dr. Aga Kosinska
CLIE, University College London

“International students found it much easier to learn new techniques using the simulations and were more confident when they got to the lab. The enhanced pre-labs resulted in students engaging with the lab manual to a greater extent than ever before.”

Details

Degrees

Biochemistry
Biomedical science
Biological science
Pharmacological science
Forensic science
Chemistry

Modules

Biological chemistry
Purification
Analytical chemistry

Topic

Chromatography

Purchase option

Chemistry LabSim Library

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