< Back to the full list


Western Blot

No items found.

Learn about the purpose of Western Blotting and the key steps involved. Explore how proteins can be transferred to a porous membrane and detected using antibodies. Practise setting up the apparatus for a wet transfer and interpreting the bands produced after blotting.

Learning Outcomes

  • Familiarise yourself with the purpose of a western blot
  • Explore the theory of the key steps
  • Learn how to set up a western blot transfer (specifically a wet transfer) and probe for a particular protein
No items found.


Concepts Principles and Theories

  • How antibodies can be used to detect the presence of target protein within a sample
  • What is happening on a molecular level during each step of the immunoblotting process

Using Instruments and Equipment

  • How to set up the blotting sandwich 
  • How to set up the transfer apparatus 
  • How to label the membrane with primary and secondary antibodies 
  • How to detect the presence of bound antibodies 
  • Why the order of the blotting sandwich components is important
  • Why the orientation of the blotting sandwich in relation to the electrodes is important 
  • Why buffer, a stir bar and an ice pack should be included in the transfer apparatus

Obtaining and Recording Data

  • Why including blocking and wash steps improves the sensitivity of the technique 
  • Why a loading control is required 

Designing and Planning Experiments

  • How to choose the right secondary antibody to pair with the primary antibody

Interpreting and Analysing Data

  • How the results of a western blot can be used to confirm the presence of a protein in a mixture of proteins 
  • How a western blot can be used to compare the level of protein between samples
Learn more about our approach to skills
Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."



Biomedical Science
Biological Science
Pharmacological Science
Forensic Science


Biological Chemistry



Purchase option

Bioscience LabSim Library

Related products

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope