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Cell Culture

Cell Counting: Haemocytometer

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Learn how to use a Neubauer haemocytometer to perform a viable cell count. Practise assembling the equipment, preparing and loading the sample, counting the number of cells and calculating the viable cell density and percentage viability.

Learning Outcomes

  • Prepare and load the sample into the haemocytometer
  • Count the number of cells in a sample
  • Manipulate the data to calculate cell density and cell viability
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Using Instruments and Equipment

  • How a haemocytometer can be used to count cells
  • How to affix the coverslip 
  • How to load the sample 
  • How to count cells

Obtaining and Recording Data

  • Why it is important to clean the equipment prior to counting
  • Why care must be taken when loading the sample into the chamber
  • Why it is important to mix the sample prior to counting
  • How trypan blue can be used to differentiate between viable and non-viable cells

Manipulating and Presenting Data

  • How to calculate the viable cell density of the original sample
  • How to calculate the percentage viability of the original sample

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Cell Culture

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