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Flow Cytometry: Fundamentals

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Learn about the internal components of a flow cytometer. Explore how differences in the morphology and protein expression of cells affect the light signals generated and allow for the characterisation of cell populations. Practise identifying and gating cell populations from dot plots.

Learning Outcomes

  • Explore the different components of a flow cytometer and how light signals are collected
  • Understand the different parameters that can be analysed using flow cytometry
  • Familiarise yourself with common data presentation and data analysis strategies
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Designing and Planning Experiments

  • How to configure the optics to split out light of the desired wavelength

Using Instruments and Equipment

  • How the laser, fluidics, optics, detectors and electronics of a flow cytometer together measure light signals  
  • What types of light signal are measured by a flow cytometer and what information they provide

Concepts Principles and Theories

  • How scattered and fluorescent light signals are generated and recorded 
  • What is meant by forward scatter, side scatter and granularity 
  • How the size and granularity of a cell affect the scattered light signals

Interpreting and Analysing Data

  • How to interpret dot plots to distinguish between cell populations 
  • How gating can be used to compare many different parameters and isolate populations of interest

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