Cytometry

Flow cytometry: fundamentals

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Learn about the internal components of a flow cytometer. Explore how differences in the morphology and protein expression of cells affect the light signals generated and allow for the characterisation of cell populations. Practise identifying and gating cell populations from dot plots.

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Learning outcomes

Explore the different components of a flow cytometer and how light signals are collected.
Understand the different parameters that can be analysed using flow cytometry.
Familiarise yourself with common data presentation and data analysis strategies.

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Skills

Designing and Planning Experiments

How to configure the optics to split out light of the desired wavelength

Using Instruments and Equipment

How the laser, fluidics, optics, detectors and electronics of a flow cytometer together measure light signals
What types of light signal are measured by a flow cytometer and what information they provide

Concepts, Principles and Theories

How scattered and fluorescent light signals are generated and recorded
What is meant by forward scatter, side scatter and granularity
How the size and granularity of a cell affect the scattered light signals

Interpreting and Analysing Data

How to interpret dot plots to distinguish between cell populations
How gating can be used to compare many different parameters and isolate populations of interest

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Prof Nicky King

University of Exeter

“Over 95% [of students] said they’d recommend them to a friend and that they wanted these resources across all the modules they were studying. So, they clearly had a really positive impact on the student experience.”

Details

Degrees

Biological science

Biomedical science

Discipline

Tissue and cell biology

Topic

Cytometry

Purchase option

Bioscience LabSim Library

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