Cytology

Cell culture: experimental application

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Use fundamental cell culture principles and physiological conditions required for cell growth. Calculate seeding parameters, antibody dilutions, and fixative concentrations to set up immunocytochemistry (ICC) experiments. Apply knowledge of ICC workflows to select suitable experimental components, troubleshoot protocol errors from confocal microscopy imaging, and evaluate quantitative data using statistical testing to form reliable conclusions.

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Learning outcomes

recall fundamental principles and practical skills in cell culture.
calculate and select appropriate parameters for ICC experimental setup.
apply knowledge of ICC workflows to identify key procedural steps and choose suitable experimental components for achieving specific aims.
analyse ICC results to determine potential sources of error within the protocol.
interpret and evaluate experimental data, select suitable statistical approaches and explain the importance of experimental repeats in producing reliable conclusions.

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Skills

Manipulating and Presenting Data

Calculate total cell yields from haemocytometer grid counts, seeding volume ratios, serial antibody dilutions, and stock fixative concentrations
Plot fluorescing cell counts and distribution graphs to evaluate data normality and variance homogeneity

Designing and Planning Experiments

Formulate testable hypotheses and design ICC workflows to evaluate drug effects on cellular targets
Select appropriate dish types, blocking reagents, primary/secondary antibody combinations, and serial dilutions to optimise experimental parameters

Using Instruments and Equipment

Utilise haemocytometers to accurately quantify cell concentrations in stock solutions
Determine and execute accurate equipment parameters for confocal microscopy imaging and fluorescence detection

Interpreting and Analysing Data

Examine confocal microscopy images to deduce procedural errors and troubleshooting artifacts in ICC protocols
Evaluate cell count distributions, select appropriate parametric or non-parametric statistical tests, and interpret p-values to draw valid conclusions

Concepts, Principles and Theories

Recall fundamental cell culture principles and human physiological conditions required for cell survival
Apply theoretical concepts of immunocytochemistry (ICC) to explain cell fixation, permeabilisation, and specific antibody-antigen interactions

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Dr. Alexandra Moores

University of Kent

"The Smart Worksheets are extremely useful to see the overall understanding and application of theories that we expected students to be able to use. Feedback is clear and accessible, enabling students to gain a deeper understanding of the topic they are studying and aiding in their success.”

Details

Degrees

Biological science

Biomedical science

Discipline

Tissue and cell biology

Topic

Cytology

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