Cytology

Cell culture: fundamentals

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Specify the roles of major cell culture media components and identify aseptic workflows required to prevent biological contamination. Calculate total cell concentration and percentage viability using trypan blue dye and a haemocytometer. Estimate cell confluency and execute core subculturing practices, including passaging, cryopreservation, and thawing procedures.

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Learning outcomes

specify the role of major components of cell culture media.
identify the aseptic workflow essential for successful cell culture.
calculate the number of cells using a haemocytometer.
complete the key processes in cell culture, including passaging, freezing and thawing of cells.

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Skills

Manipulating and Presenting Data

Calculate percentage cell viability, total cell concentrations, and dilution factors from raw grid counts
Determine exact cell suspension and media volumes required to achieve target seeding densities for experimental plates

Obtaining and Recording Data

Collect counts of live and dead cells across designated haemocytometer quadrants using trypan blue dye exclusion
Document visual indicators of cell confluency, culture status, and experimental counts systematically

Using Instruments and Equipment

Operate biosafety cabinets correctly to maintain sterile technique and protect cell cultures from airborne pathogens
Utilise haemocytometers and optical microscopes to perform cell counts and assess cellular morphology

Interpreting and Analysing Data

Evaluate changes in media color and microscopic imagery to estimate confluency and detect culture contamination
Analyse experimental variables to assess the impact of improper trypsinization or cryoprotectant exposure on cell health

Concepts, Principles and Theories

Identify the roles of essential cell culture media components and aseptic workflows required to prevent contamination
Apply biological principles to explain the mechanisms of trypsin dissociation, cell passaging, and cryopreservation

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Balgisa, Pharmaceutical Sciences Student, London Metropolitan University

"I found using Smart Worksheets easy and clear. The lecturers helped us to understand how to work with them. They gave us a lot of examples and questions, so we practised a lot through our course. When the exam took place, we all were confident enough to pass this part of the exam."

Details

Degrees

Biological science

Biomedical science

Discipline

Tissue and cell biology

Topic

Cytology

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