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Light Microscopy

Estimating Size

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Learn how to calculate the total magnification of a microscope when three different objective lenses are used in combination with a 10x ocular lens. Practise calibrating an eyepiece graticule using a stage micrometer and measuring the size of an onion epidermal cell.

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Learning Outcomes

  • Calculate the total magnification of an image when different objective lenses are used with the same eyepiece
  • Familiarise yourself with how a stage micrometer is used to calibrate an eyepiece graticule
  • Use an eyepiece graticule to measure the size of 3 different objects seen down the microscope
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Skills

Using Instruments and Equipment

  • Why the eyepiece graticule requires calibration at different magnifications 
  • How to calibrate the eyepiece graticule with a stage micrometer

Obtaining and Recording Data

  • How to line up an object with the scale of the eyepiece graticule 
  • How to use an eyepiece graticule to measure the size of a object viewed down the microscope

Concepts Principles and Theories

  • What lenses contribute to the magnification of an image in a compound light microscope 
  • How to calculate the total magnification of an image when different objective lenses are in use 

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“I find using LabSims really helpful as preparation before a practical. It's useful to be able to carry out some of the practical techniques online before completing them in the lab, and they make me feel more confident about attempting these techniques during our lab sessions.”

Details

Degrees

Biochemistry
Biological Science
Biomedical Science
Forensic Science
Pharmacological Science

Modules

Anatomy & Physiology
Tissue & Cell Biology
Microbiology
Ecology & Evolution
Immunology
Forensic Investigation

Topic

Light Microscopy

Purchase option

Bioscience LabSim Library

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Online, autograded assessment tool for advanced data analysis and calculation practice
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