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Light Microscopy

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Learn how to calculate the total magnification of a microscope when three different objective lenses are used in combination with a 10x ocular lens. Practise calibrating an eyepiece graticule using a stage micrometer and measuring the size of an onion epidermal cell.

Learning Outcomes

  • Calculate the total magnification of an image when different objective lenses are used with the same eyepiece
  • Familiarise yourself with how a stage micrometer is used to calibrate an eyepiece graticule
  • Use an eyepiece graticule to measure the size of 3 different objects seen down the microscope
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Using Instruments and Equipment

  • Why the eyepiece graticule requires calibration at different magnifications 
  • How to calibrate the eyepiece graticule with a stage micrometer

Obtaining and Recording Data

  • How to line up an object with the scale of the eyepiece graticule 
  • How to use an eyepiece graticule to measure the size of a object viewed down the microscope

Concepts Principles and Theories

  • What lenses contribute to the magnification of an image in a compound light microscope 
  • How to calculate the total magnification of an image when different objective lenses are in use 

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Light Microscopy

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