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Gel Filtration: Separating Proteins by Size

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Learn how the composition of the column gel facilitates the separation of proteins based on their molecular size. Practise predicting the relative elution speed and elution order of proteins of different sizes.

Learning Outcomes

  • Explore how different sized proteins pass through or around gel beads in a column
  • Consider how differences in protein size can be used to aid protein purification
  • Predict the elution order of different sized proteins when run through a gel filtration column
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Concepts Principles and Theories

  • What is the composition of the gel used in the column
  • How protein size affects migration through the gel
  • How to predict the order of elution of different sized proteins

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