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Haematoxylin & Eosin (H&E) Stain

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Learn about the reagents, mechanism and steps involved in a principal histological stain. Explore the mechanism of staining by the two dyes involved in the process, and rearrange the steps of the protocol to optimise the staining. Identify the correct order of reagents and the role of certain reagents in the protocol.

Learning outcomes

  • Describe the mechanism of staining by the two dyes
  • List the order and role each reagent plays in the staining protocol
  • Troubleshoot the protocol to optimise staining
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Concepts, Principles and Theories

  • What is the purpose of a H&E stain 
  • Which cellular structures are stained by Haematoxylin and Eosin 
  • What is the role of the different reagents used in a H&E stain 

Using Instruments and Equipment

  • How to prepare the tissue for staining 
  • How to stain the tissue with H&E 
  • How to prepare the tissue for coverslipping 
  • Why the solutions for each phase of the staining process must be applied in a specific order 

Obtaining and Recording Data

  • What effect altering the time in the staining solutions has on the quality of the final result
  • How best to adjust the protocol to correct for some common issues with the H&E stain

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