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Staining Blood Smear: Leishman's Stain

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Learn about the equipment and reagents used in the Leishman's staining protocol, and the effect of the stain on different cell types. Identify understained, overstained and appropriately stained blood smears.

Learning outcomes

  • Familiarise yourself with the equipment involved in staining a blood smear
  • Assemble a flow chart for staining using Leishman’s stain
  • Identify when a smear is overstained or understained, as seen down a microscope
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Designing and Planning Experiments

  • What are the advantages of using a Coplin jar as opposed to a Pasteur pipette to apply staining solutions

Concepts, Principles and Theories

  • What is the purpose of preparing and staining a blood smear

Using Instruments and Equipment

  • What are the steps and reagents involved in performing a Leishman’s stain

Obtaining and Recording Data

  • What is the effect of over or under exposing the blood smear to stain

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Dr. Sharon Williams
Coventry University

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