< Back to the full list

Chromatography

Ion Exchange Chromatography

No items found.

Learn the key principles of ion exchange chromatography, and the relationship between pH, protein and isoelectric points (pl). Examine an ion exchange column to predict which proteins will bind or elute under differing pH conditions.

Request a demo
See skills
arrow_downward

Learning Outcomes

  • Consider the relationship between pH, protein isoelectric points (pI) and protein charge
  • Explore how differences in pI of proteins can be used to aid protein purification
  • Predict protein binding or elution to anion and cation exchange columns under different pH conditions
No items found.
chevron_left
chevron_right

Skills

Concepts Principles and Theories

  • What is the relationship between pH and overall charge on a protein
  • What is meant by the isoelectric point
  • How differences in the isoelectric point of proteins can be used to aid protein purification
  • How to predict whether a protein will bind to the column equilibrated at a particular pH when given its isoelectric point
  • How changing the buffer added to the column affects the pH environment and elution of proteins

Designing and Planning Experiments

  • What is the difference between a cation and anion exchange column

Interpreting and Analysing Data

  • How to identify the order proteins will reach their isoelectric point when given a graph of charge vs pH

Learn more about our approach to skills
Student response from Students Insights Survey

“I like that it goes through all the steps, so you have a better understanding of what you have to do for the actual lab... It makes me feel more prepared.”

Details

Degrees

Biochemistry
Biomedical Science
Biological Science
Pharmacological Science
Forensic Science
Chemistry

Modules

Biological Chemistry
Purification
Analytical Chemistry

Topic

Chromatography

Purchase option

Bioscience LabSim Library

Related products

Chromatography

High Performance Liquid Chromatography (HPLC)

LabSim

Chromatography

Size Exclusion Chromatography

LabSim

Chromatography

Column Chromatography

LabSim

Chromatography

Running a Protein Purification Column

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope