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Enzyme Kinetics

Lineweaver-Burk Plot

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Learn how the Lineweaver-Burk plot can be used to determine the type of inhibition on an enzyme. Explore the relationship between the equation and plot and practise transforming Michaelis-Menten data, generating a Lineweaver-Burk plot and estimating KM and Vmax.

Learning Outcomes

  • Identify key features of a Lineweaver-Burk plot, including axes labels and intercept values
  • Transform Michaelis-Menten data into a form suitable for Lineweaver-Burk plotting
  • Use LWB plots to estimate KM and Vmax, and determine the effect of different inhibitors
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Manipulating and Presenting Data

  • How to transform Michaelis-Menten data into a form suitable for Lineweaver-Burk plotting

Interpreting and Analysing Data

  • How do the gradient, intercepts and axes of a Lineweaver-Burk plot relate to the components in the equation 
  • How to estimate the Vmax and Km from a Lineweaver-Burk plot
  • What effect competitive and non-competitive inhibitors have on the KM and Vmax values

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Enzyme Kinetics

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