Protein analysis techniques

Protein purification and yield

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Perform calculations involved in protein purification. Analyse experimental results from hypothetical students, calculating concepts like total/specific activity, percent yield and purification factor.

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Learning outcomes

Define 'total activity', 'percent recovery', 'specific activity' and 'purification factor'.
Calculate the total activity and the percent recovery for a cell extract and purified sample.
Calculate the specific activity and the purification factor for a cell extract and purified sample.
Evaluate experimental purification data to determine relative success.

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Skills

Concepts, Principles and Theories

How to apply total activity and specific activity in context
How to differentiate percent recovery and purification factor
How to determine level of success of purification for outlined objective

Interpreting and Analysing Data

How to calculate total activity and specific activity
How to calculate percent recovery from crude product
How to calculate purification factor of extracted product

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Balgisa, Pharmaceutical Sciences Student, London Metropolitan University

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Details

Degrees

Biological science

Biomedical science

Foundation science

Modules

Molecular biology

Topic

Protein analysis techniques

Purchase option

The Smart Worksheet Library

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