Immunodetection

Sandwich ELISA

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Learn about the reagents and steps involved in an ELISA, focusing on sandwich ELISAs. Review the protocol order and understand the relevant controls used in an ELISA.

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Learning outcomes

Learn about the reagents and steps involved in an ELISA
Answer questions about the theory of the ELISA
Familiarise yourself with the relevant controls used in an ELISA

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Skills

Concepts, Principles and Theories

How antibodies can be used to detect the presence of an analyte within a sample
What is happening on a molecular level during each step of the ELISA
What it means to immobilise the antigen and why this is necessary
What is meant by a colorimetric reaction
How the results of an ELISA can be used to estimate the concentration of an analyte in a sample

Using Instruments and Equipment

What are the core steps and components of an ELISA
How to immobilise the antigen
How to probe for the presence of antigen
How to detect bound antigen

Obtaining and Recording Data

Why including blocking and wash steps increases the sensitivity of the assay
Why samples, standards and controls should be assayed in duplicate or triplicate

Designing and Planning Experiments

What controls should be assayed alongside samples in an ELISA

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PGR student, School of Pharmacy

"I accessed the Sandwich-ELISA resource from the PGR-Biosciences-Lab-Shots library as face-to-face training was to be avoided during Covid-19-restrictions. As a result, I felt much more confident when discussing the technique with my supervisor and undertaking ELISAs for the first time on my own in the lab."

Details

Degrees

Biological science

Biomedical science

Discipline

Molecular biology

Topic

Immunodetection

Purchase option

Bioscience LabSim Library

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