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Immunodetection

Sandwich ELISA

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Learn about the reagents and steps involved in an ELISA, focusing on sandwich ELISAs. Review the protocol order and understand the relevant controls used in an ELISA.

Learning outcomes

  • Learn about the reagents and steps involved in an ELISA
  • Answer questions about the theory of the ELISA
  • Familiarise yourself with the relevant controls used in an ELISA
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Skills

Concepts, Principles and Theories

  • How antibodies can be used to detect the presence of an analyte within a sample
  • What is happening on a molecular level during each step of the ELISA 
  • What it means to immobilise the antigen and why this is necessary 
  • What is meant by a colorimetric reaction 
  • How the results of an ELISA can be used to estimate the concentration of an analyte in a sample

Using Instruments and Equipment

  • What are the core steps and components of an ELISA 
  • How to immobilise the antigen 
  • How to probe for the presence of antigen 
  • How to detect bound antigen

Obtaining and Recording Data

  • Why including blocking and wash steps increases the sensitivity of the assay 
  • Why samples, standards and controls should be assayed in duplicate or triplicate

Designing and Planning Experiments

  • What controls should be assayed alongside samples in an ELISA

Learn more about our approach to skills
PGR student, School of Pharmacy

"I accessed the Sandwich-ELISA resource from the PGR-Biosciences-Lab-Shots library as face-to-face training was to be avoided during Covid-19-restrictions. As a result, I felt much more confident when discussing the technique with my supervisor and undertaking ELISAs for the first time on my own in the lab."

Details

Degrees

Biochemistry
Biological science
Biomedical science
Forensic science
Pharmacological science

Modules

Immunology
Pharmacology

Topic

Immunodetection

Purchase option

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