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Enzyme kinetics

Michaelis-Menten Plot

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Learn the main features of the Michaelis-Menten equation and Michaelis-Menten plots. Generate and analyse a Michaelis-Menten plot to determine Vmax and KM for an enzyme-catalysed reaction.

Learning outcomes

  • Define all components of the Michaelis-Menten equation
  • Select appropriate data collection values for generating a Michaelis-Menten plot
  • Estimate Vmax and KM values from a Michaelis-Menten plot
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Concepts, Principles and Theories

  • What does the Michaelis-Menten equation and corresponding plot describe
  • What are the components of the Michaelis-Menten equation and what do they describe

Designing and Planning Experiments

  • How to choose a sensible range of substrate concentrations to test

Interpreting and Analysing Data

  • How to estimate the Vmax and KM from a Michaelis-Menten plot

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Enzyme kinetics

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