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Enzyme kinetics

Michaelis-Menten plot

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Learn the main features of the Michaelis-Menten equation and Michaelis-Menten plots. Generate and analyse a Michaelis-Menten plot to determine Vmax and KM for an enzyme-catalysed reaction.
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Learning outcomes

  • Define all components of the Michaelis-Menten equation.
  • Select appropriate data collection values for generating a Michaelis-Menten plot.
  • Estimate <i>V</i><sub>max</sub> and <i>K</i><sub>M</sub> values from a Michaelis-Menten plot.
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Skills

Concepts, Principles and Theories

  • What does the Michaelis-Menten equation and corresponding plot describe
  • What are the components of the Michaelis-Menten equation and what do they describe

Designing and Planning Experiments

  • How to choose a sensible range of substrate concentrations to test

Interpreting and Analysing Data

  • How to estimate the Vmax and KM from a Michaelis-Menten plot

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Altantic Technological University

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Details

Degrees

Biological science
Biomedical science
Foundation science
Pharmaceutical science

Discipline

Molecular biology

Topic

Enzyme kinetics

Purchase option

Bioscience LabSim Library

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Biological science
Biomedical science
Foundation science
Pharmaceutical science
enzyme kinetics
Molecular biology
Bioscience LabSim Library
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