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Culture of Microorganisms

Pour Plate Technique

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Learn how to prepare a pour plate by following a stepwise virtual lab protocol. Explore good aseptic technique, flame sterilisation and using an automated pipette to dispense bacteria. Review the results of the pour plate and the reasoning behind decisions taken throughout.

Learning Outcomes

  • Familiarise yourself with the equipment required to prepare a pour plate
  • Outline the key steps involved in making a pour plate and understand the reasoning behind each step
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Using Instruments and Equipment

  • What equipment is required to perform the pour plate technique
  • Why plates should be labelled on their base and what it should detail
  • How to flame the neck of the culture and agar bottle 
  • How to transfer the inoculum and molten agar into a Petri dish
  • Why working under a bunsen burner and flaming the neck of the bottles helps reduce contamination
  • How to handle the equipment to reduce the risk of contamination 

Obtaining and Recording Data

  • Why plates should be placed upside down in the incubator 
  • Why the plate should be gently swirled to mix the agar and the inoculum

Working in a Safe Manner

  • Why care must be taken when flaming the neck of the bottles
  • Why care must be taken when pipetting the inoculum and pouring the agar into the Petri dish

Concepts Principles and Theories

How the appearance of colonies differs between those growing within the agar and those growing on the surface

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Culture of Microorganisms

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