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Culture of microorganisms

Streak Plate Technique

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Learn how to prepare a streak plate by following a stepwise virtual lab protocol. Explore good aseptic techniques and how to achieve the optimal streak technique.

Learning outcomes

  • Familiarise yourself with the equipment required to prepare a streak plate
  • Outline the key steps of making a streak plate and understand the reasoning behind each step
  • Identify single colonies growing on a streak plate for further analysis
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Skills

Using Instruments and Equipment

  • What equipment is required to streak bacteria on an agar plate
  • Why plates should be labelled on their base and what it should detail
  • How to flame the neck of the culture bottle and inoculation loop
  • How to streak a bacterial culture on an agar plate
  • Why working under a bunsen burner and flaming the neck of the culture bottle helps reduce contamination
  • How to handle the equipment to reduce the risk of contamination 
  • Why the sterile loop should not be used to spread bacteria while it is hot 

Obtaining and Recording Data

  • What streak pattern is used to achieve discrete colonies
  • Why plates should be placed upside down in the incubator
  • How the density of bacterial colonies differs in different parts of the streak plate

Working in a Safe Manner

  • Why care must be taken when flaming the neck of the bottle
  • How to reduce the risk of burning yourself when sterilising the inoculation loop

Learn more about our approach to skills
Student
University of Exeter

“The feedback from the simulations was really useful to understand where I went wrong, so I don’t go wrong when I am doing the lab in real time.”

Details

Degrees

Biochemistry
Biomedical science
Biological science
Forensic science
Pharmacological science

Modules

Microbiology

Topic

Culture of microorganisms

Purchase option

Bioscience LabSim Library

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