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Culture of Microorganisms

Pouring an Agar Plate

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Learn about the equipment and steps involved in pouring an agar plate. Explore good aseptic technique, including Bunsen burner use, flame sterilisation and lid positioning.

Learning Outcomes

  • Familiarise yourself with the equipment needed to pour an agar plate
  • Use good aseptic technique to pour an agar plate
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Using Instruments and Equipment

  • What equipment is required to pour an agar plate
  • How to flame the neck of the bottle 
  • Why working under a bunsen burner and flaming the neck of the agar bottle helps reduce contamination
  • How to handle the lids of containers to reduce the risk of contamination 
  • How to dry and store the agar plate

Working in a Safe Manner

  • Why care must be taken when flaming the neck of the bottle  
  • Why care must be taken when pouring the agar into the Petri dish

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Dr Anna Maria Piccinini
University of Nottingham

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Culture of Microorganisms

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