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Culture of microorganisms

Pouring an Agar Plate

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Learn about the equipment and steps involved in pouring an agar plate. Explore good aseptic technique, including Bunsen burner use, flame sterilisation and lid positioning.

Learning outcomes

  • Familiarise yourself with the equipment needed to pour an agar plate
  • Use good aseptic technique to pour an agar plate
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Skills

Using Instruments and Equipment

  • What equipment is required to pour an agar plate
  • How to flame the neck of the bottle 
  • Why working under a bunsen burner and flaming the neck of the agar bottle helps reduce contamination
  • How to handle the lids of containers to reduce the risk of contamination 
  • How to dry and store the agar plate

Working in a Safe Manner

  • Why care must be taken when flaming the neck of the bottle  
  • Why care must be taken when pouring the agar into the Petri dish

Learn more about our approach to skills
Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"

Details

Degrees

Biochemistry
Biomedical science
Forensic science
Pharmacological science
Biological science

Modules

Molecular biology & genetics
Microbiology

Topic

Culture of microorganisms

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