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Chromatography

Running a Protein Purification Column

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Learn how to properly use a protein purification column by exploring the main practical considerations when running one. See a labelled diagram of a protein purification column and practise separating a mix of two proteins using column chromatography.

Learning Outcomes

  • Explore the components of and key practical points to consider in a purification column
  • Add a protein mixture with appropriate care and suitable technique
  • Successfully separate and purify the protein mixture in appropriate vessels
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Skills

Using Instruments and Equipment

  • What are the main features of a purification column
  • How to operate the column tap to start and stop the flow of elution
  • What is used to equilibrate the column prior to adding the protein mixture

Obtaining and Recording Data

  • Why the gel bed must not be allowed to dry
  • Why care must be taken when pipetting the sample into the top of the column
  • Why it is important to keep the gel bed flat
  • When to start and stop collecting each protein as they elute

Learn more about our approach to skills
Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"

Details

Degrees

Biochemistry
Biomedical Science
Biological Science
Pharmacological Science
Forensic Science
Chemistry

Modules

Biological Chemistry
Purification
Analytical Chemistry

Topic

Chromatography

Purchase option

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