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Column chromatography

Running a protein purification column

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Learn how to properly use a protein purification column by exploring the main practical considerations when running one. See a labelled diagram of a protein purification column and practise separating a mix of two proteins using column chromatography.
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Learning outcomes

  • Explore the components of and key practical points to consider in a purification column.
  • Add a protein mixture with appropriate care and suitable technique.
  • Successfully separate and purify the protein mixture in appropriate vessels.
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Skills

Using Instruments and Equipment

  • What are the main features of a purification column
  • How to operate the column tap to start and stop the flow of elution
  • What is used to equilibrate the column prior to adding the protein mixture

Obtaining and Recording Data

  • Why the gel bed must not be allowed to dry
  • Why care must be taken when pipetting the sample into the top of the column
  • Why it is important to keep the gel bed flat
  • When to start and stop collecting each protein as they elute

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Dr Anna Maria Piccinini
University of Nottingham

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Details

Degrees

Biological science
Biomedical science
Foundation science

Discipline

Molecular biology

Topic

Column chromatography

Purchase option

Bioscience LabSim Library

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Biological science
Biomedical science
Foundation science
chromatography
Molecular biology
Bioscience LabSim Library
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