< Back to the full list


Running a Protein Purification Column

No items found.

Learn how to properly use a protein purification column by exploring the main practical considerations when running one. See a labelled diagram of a protein purification column and practise separating a mix of two proteins using column chromatography.

Learning Outcomes

  • Explore the components of and key practical points to consider in a purification column
  • Add a protein mixture with appropriate care and suitable technique
  • Successfully separate and purify the protein mixture in appropriate vessels
No items found.


Using Instruments and Equipment

  • What are the main features of a purification column
  • How to operate the column tap to start and stop the flow of elution
  • What is used to equilibrate the column prior to adding the protein mixture

Obtaining and Recording Data

  • Why the gel bed must not be allowed to dry
  • Why care must be taken when pipetting the sample into the top of the column
  • Why it is important to keep the gel bed flat
  • When to start and stop collecting each protein as they elute

Learn more about our approach to skills
Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"



Biomedical Science
Biological Science
Pharmacological Science
Forensic Science


Biological Chemistry
Analytical Chemistry



Purchase option

Bioscience LabSim Library

Related products

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope