Spectrometry and spectrophotometry

UV-Vis calibration curves

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Explore how a UV-Vis spectrometer can be used to measure the absorbance of a sample and learn how to use the data to calculate concentrations and absorption coefficients. Plot calibration curves to find unknown concentrations from absorbance values and apply this to various environmental chemistry scenarios.

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Learning outcomes

Identify the steps involved in recording a UV-Vis spectrum.
Use the Beer-Lambert equation to calculate absorption coefficients and absorbance.
Construct and use calibration curves to determine unknown concentrations of chemical compounds.

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Skills

Using Instruments and Equipment

Describing how a spectrophotometer works
Identifying the correct order of steps when running a sample for UV-Vis
How to take an accurate reading using a UV-Vis spectrophotometer

Manipulating and Presenting Data

How to select the data required to construct a calibration curve
How to use a calibration curve to calculate absorption coefficients and concentrations of unknown samples

Interpreting and Analysing Data

How to use the Beer-Lambert law to calculate absorbances, absorption coefficents and concentrations based on experimental data

Concepts, Principles and Theories

Identifying the components of the Beer-Lambert equation
Explaining the limitations of the Beer-Lambert law

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Prof. Dudley Shallcross

Professor of Atmospheric Chemistry, University of Bristol

“Students welcome the opportunity to repeat calculations with different data to test their understanding of the material. Student feedback on courses that I teach that use Smart Worksheets, always highlight this as a very positive aspect.”

Details

Degrees

Pharmaceutical science

Forensic science

Chemistry

Modules

Analytical chemistry

Topic

Spectrometry and spectrophotometry

Purchase option

The Smart Worksheet Library

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