< Back to the full list

Molecular Cloning

Blue-White Screening Theory

No items found.

Learn the molecular basis of blue-white screening, including the role of the lac operon, beta-galactosidase and alpha-complementation. Explore how the success of ligation and transformation steps affects the growth and appearance of bacteria on the plate and practise identifying colonies containing recombinant bacteria.

Learning Outcomes

  • Explore the function and molecular basis of blue-white screening
  • Determine the role of each reagent
  • Explain key results in terms of transformation success
No items found.


Obtaining and Recording Data

  • Why the agar must be supplemented with the appropriate antibiotic, IPTG and X-gal

Interpreting and Analysing Data

  • How to identify colonies that have taken up the plasmid with the desired insert

Concepts Principles and Theories

  • What is the purpose of blue-white screening
  • What components make up the lac operon in E. coli
  • Why IPTG is seen as an activator of the lac operon
  • Why bacteria that have not taken up the plasmid die before forming colonies
  • Why bacteria that have taken up the plasmid with no insert will form blue colonies
  • Why bacteria that have taken up the plasmid with insert will form white colonies

Learn more about our approach to skills
Student, Natural Sciences
University of Exeter

“I really like labs but it can be challenging because the apparatus is new and very different to school. I use LabSims during the week on my laptop and make notes the night before a lab. Seeing it in the simulation then the lab made it more familiar and helped me feel like I knew what I was doing. I could make mistakes in the simulation and learn from that first.”



Biomedical Science
Biological Science
Pharmacological Science
Forensic Science


Molecular Biology & Genetics


Molecular Cloning

Purchase option

Bioscience LabSim Library

Related products

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope