Learn the molecular basis of blue-white screening, including the role of the lac operon, beta-galactosidase and alpha-complementation. Explore how the success of ligation and transformation steps affects the growth and appearance of bacteria on the plate and practise identifying colonies containing recombinant bacteria.
“I really like labs but it can be challenging because the apparatus is new and very different to school. I use LabSims during the week on my laptop and make notes the night before a lab. Seeing it in the simulation then the lab made it more familiar and helped me feel like I knew what I was doing. I could make mistakes in the simulation and learn from that first.”