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Molecular cloning

Cloning and Expression Plasmids

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Learn about the key elements of cloning and expression plasmids. Explore the process of transcription and translation of a gene of interest inserted into an expression plasmid, and practise designing suitable cloning and expression plasmids for experiments.

Learning outcomes

  • Label the different parts of a cloning plasmid
  • Correctly order the parts of an expression plasmid
  • Read through a set of cloning scenarios, and design a plasmid suitable for each experiment
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Skills

Designing and Planning Experiments

  • What experimental factors must be considered when designing an appropriate plasmid

Concepts, Principles and Theories

  • How different elements found in cloning plasmids facilitate the insertion, replication and selection of DNA
  • How components within an expression plasmid facilitate the transcription and translation of a protein of interest

Learn more about our approach to skills
Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"

Details

Degrees

Biomedical science
Biological science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Molecular cloning

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