Cloning

Cloning overview

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Learn the key steps involved in gene cloning. Explore and examine the process from initial ligation of the insert into a plasmid to using agarose gel electrophoresis to confirm successful cloning.

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Learning outcomes

Excise an insert and ligate it into a recipient plasmid to make a recombinant plasmid.
Transform competent cells with recombinant plasmid.
Select for cells that have taken up plasmid.
Confirm that the cloning process was successful.

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Skills

Concepts, Principles and Theories

What processes are utilised to insert a desired DNA segment into a recipient plasmid
How restriction enzyme digests are used to cut out the desired insert and linearize the recipient plasmid
How DNA ligase is used to ligate the insert into the recipient plasmid
How transformation of bacterial cells produces more copies of plasmid DNA
What is meant by a competent bacterial cells
How selection is used to remove cells that have not taken up plasmid
How agarose gel electrophoresis can be used to analyse the success of the cloning

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Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Cloning

Purchase option

Bioscience LabSim Library

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