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Molecular Cloning

Cloning: Overview

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Learn the key steps involved in gene cloning. Explore and examine the process from initial ligation of the insert into a plasmid to using agarose gel electrophoresis to confirm successful cloning.

Learning Outcomes

  • Excise an insert and ligate it into a recipient plasmid to make a recombinant plasmid
  • Transform competent cells with recombinant plasmid
  • Select for cells that have taken up plasmid
  • Confirm that the cloning process was successful
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Concepts Principles and Theories

  • What processes are utilised to insert a desired DNA segment into a recipient plasmid
  • How restriction enzyme digests are used to cut out the desired insert and linearize the recipient plasmid 
  • How DNA ligase is used to ligate the insert into the recipient plasmid 
  • How transformation of bacterial cells produces more copies of plasmid DNA 
  • What is meant by a competent bacterial cells 
  • How selection is used to remove cells that have not taken up plasmid 
  • How agarose gel electrophoresis can be used to analyse the success of the cloning

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Molecular Cloning

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