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Molecular Cloning

Miniprep 2: Purify Plasmid DNA

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Learn the key steps involved in purifying plasmid DNA in a miniprep protocol. Test your knowledge and understanding with interactive animations and questions.

Learning Outcomes

  • Use a spin column with a silica membrane to bind the plasmid DNA
  • Homogenise the lysate and add it to a silica spin column
  • Wash the column to remove impurities
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Using Instruments and Equipment

  • How the features of a miniprep column facilitate the purification of plasmid DNA

Obtaining and Recording Data

  • Which buffers are required to wash the membrane and elute the plasmid DNA 
  • Why it is important to wash the membrane

Concepts Principles and Theories

  • What is happening on a molecular level during each step of the purification process

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Dr Sheila Faherty
Altantic Technological University

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Molecular Cloning

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