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Column Chromatography

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Learn and familiarise yourself with a column chromatography setup. Explore the results of adjusting the solvent volume, fraction size and column height, and determine the optimum set-up.

Learning Outcomes

  • Familiarise yourself with column chromatography setup
  • Adjust parameters such as solvent volume, fraction size and column height
  • Understand the consequences of choices made on the separationLearn how to determine the optimum setup for column chromatography
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Using Instruments and Equipment

  • Why the solvent should be kept topped up 
  • Why a layer of sand should be included at the top and the base of the column
  • Why a cotton or glass wool plug should be included at the base of the column
  • Why it is advisable to use a small size of collection tube

Obtaining and Recording Data

  • How to ensure the band of sample is concentrated in the column 
  • How the column height affects the separation of components

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