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Nucleic Acid Quantification

DNA Quantification

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Learn how to compare and use NanoDrop and Qubit technology, explore DNA, salt and protein spectra, and measure DNA using a fluorometer. Understand the pros and cons of each technology and interpret the results.

Learning Outcomes

  • Explain how DNA can be quantified and contamination detected using a NanoDrop
  • Explain how DNA can be quantified using a Qubit
  • Outline the pros and cons of each method
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Designing and Planning Experiments

  • How the NanoDrop and Qubit differ in terms of sample preparation, type of measurement and accuracy

Interpreting and Analysing Data

  • How to interpret a typical absorbance spectra and results from a NanoDrop
  • What 260/280 and 230/260 ratios are indicative of significant contamination

Concepts Principles and Theories

  • Why the absorbance at 260 nm is used to measure DNA concentration 
  • Why the 260/280 and 260/230 ratios indicate the level of protein and salt contamination
  • Why quantification of dsDNA using a fluorometer relies on the addition of a fluorescent dsDNA binding dye

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Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."



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Nucleic Acid Quantification

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