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Nucleic Acid Quantification

DNA Quantification

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Learn how to compare and use NanoDrop and Qubit technology, explore DNA, salt and protein spectra, and measure DNA using a fluorometer. Understand the pros and cons of each technology and interpret the results.

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Learning Outcomes

  • Explain how DNA can be quantified and contamination detected using a NanoDrop
  • Explain how DNA can be quantified using a Qubit
  • Outline the pros and cons of each method
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Skills

Designing and Planning Experiments

  • How the NanoDrop and Qubit differ in terms of sample preparation, type of measurement and accuracy

Interpreting and Analysing Data

  • How to interpret a typical absorbance spectra and results from a NanoDrop
  • What 260/280 and 230/260 ratios are indicative of significant contamination

Concepts Principles and Theories

  • Why the absorbance at 260 nm is used to measure DNA concentration 
  • Why the 260/280 and 260/230 ratios indicate the level of protein and salt contamination
  • Why quantification of dsDNA using a fluorometer relies on the addition of a fluorescent dsDNA binding dye

Learn more about our approach to skills
Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."

Details

Degrees

Biochemistry
Biomedical Science
Pharmacological Science
Forensic Science
Biological Science

Modules

Molecular Biology & Genetics
Forensic Investigation

Topic

Nucleic Acid Quantification

Purchase option

Bioscience LabSim Library

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