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Next Generation Sequencing

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Learn about the main stages of Next Generation Sequencing (NGS), how and why DNA fragments are amplified into clusters and how sequencing by synthesis occurs. Explore how a DNA sample is prepared and practise loading a sample into the Illumina MiSeq.

Learning Outcomes

  • Outline the the main stages of NGS and the key components of an NGS sequencer
  • Explain how a DNA sample is prepared and why DNA fragments are amplified into clusters
  • Describe how sequencing by synthesis happens and how base calls are made
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Concepts Principles and Theories

  • What are the main stages of next generation sequencing 
  • What is happening on a molecular level during sample preparation steps
  • Why it is important to determine the quantity and quality of DNA before starting 
  • What is the purpose of the clustering, multiplexing and sequencing regions of the adapters 
  • What is happening on a molecular level during cluster generation steps
  • Why DNA fragments must be amplified into clusters 
  • What is happening on a molecular level during the sequencing by synthesis steps

Using Instruments and Equipment

  • What are the main parts of an Illumina MiSeq

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