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SSLP Detection

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Learn about Simple Sequence Length Polymorphisms (SSLPs) and how to use PCR and agarose gel electrophoresis to isolate and visualise them. Practise predicting the size of PCR products for different alleles, identifying band patterns and creating an SSLP detection workflow.

Learning Outcomes

  • Define SSLP
  • Predict PCR products of alleles with SSLP
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Concepts Principles and Theories

  • What is meant by simple sequence length polymorphisms (SSLP’s)
  • How an SSLP affects the length of the expected PCR products

Interpreting and Analysing Data

  • How to identify the alleles present in different DNA samples using PCR and agarose gel electrophoresis

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