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Nucleic Acid Extraction

RNA Extraction from Animal Cells

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Learn about the key stages involved in the extraction of RNA from animal cells using a spin column. Explore the resuspension of the cell pellet, lysis of the cells, homogenisation of the sample and isolation of the RNA.

Learning Outcomes

  • Lyse the cell pellet, and identify the role of the lysis buffer
  • Homogenise the lysate and add it to a silica spin column
  • Complete two washing steps, followed by elution of the purified RNA
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Using Instruments and Equipment

  • How to resuspend the cell pellet in lysis buffer 
  • How the features of a miniprep column facilitate the extraction of RNA

Obtaining and Recording Data

  • Why it is important to use a low salt solution to wash residual proteins off the membrane
  • Why it is important to use ethanol to wash salt off the membrane

Concepts Principles and Theories

  • What is happening on a molecular level during the lysis, homogenisation and purification steps 
  • How do the components of the lysis and binding buffer facilitate their roles in RNA extraction

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Nucleic Acid Extraction

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