< Back to the full list

Nucleic Acid Extraction

RNA Extraction from Animal Cells

No items found.

Learn about the key stages involved in the extraction of RNA from animal cells using a spin column. Explore the resuspension of the cell pellet, lysis of the cells, homogenisation of the sample and isolation of the RNA.

Request a demo
See skills
arrow_downward

Learning Outcomes

  • Lyse the cell pellet, and identify the role of the lysis buffer
  • Homogenise the lysate and add it to a silica spin column
  • Complete two washing steps, followed by elution of the purified RNA
No items found.
chevron_left
chevron_right

Skills

Using Instruments and Equipment

  • How to resuspend the cell pellet in lysis buffer 
  • How the features of a miniprep column facilitate the extraction of RNA

Obtaining and Recording Data

  • Why it is important to use a low salt solution to wash residual proteins off the membrane
  • Why it is important to use ethanol to wash salt off the membrane

Concepts Principles and Theories

  • What is happening on a molecular level during the lysis, homogenisation and purification steps 
  • How do the components of the lysis and binding buffer facilitate their roles in RNA extraction

Learn more about our approach to skills
Grace, Biosciences Student at the University of Exeter

"Seeing it in the simulation then the lab made it more familiar and helped me feel like I knew what I was doing. I could make mistakes in the simulation and learn from that first."

Details

Degrees

Biochemistry
Biomedical Science
Biological Science
Pharmacological Science
Forensic Science

Modules

Molecular Biology & Genetics

Topic

Nucleic Acid Extraction

Purchase option

Bioscience LabSim Library

Related products

Nucleic Acid Quantification

DNA Quantification

LabSim

Sequencing

Next Generation Sequencing

LabSim

Genetic Recombination

Crossing Over and Recombination

LabSim

Genotyping

SSLP Detection

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope