Genetics

SNP detection with CAPS

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Learn about how Cleaved Amplified Polymorphic Sequences (CAPS) can be used to determine between sequences with single nucleotide polymorphisms (SNPs).

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Learning outcomes

Define SNPs and CAPS
Predict PCR products of alleles with SNPs
Predict digest patterns of alleles with SNPs

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Skills

Concepts, Principles and Theories

What is meant by a Single Nucleotide Polymorphism (SNP)
Why the CAPS method relies on the SNP falling within a restriction enzyme site
How SNPs in restriction enzyme sites affects enzymatic activity
How an SNP in a restriction enzyme site does not affect the length of the expected PCR products
How an SNP in a restriction enzyme site does affect the length of the expected digest products

Interpreting and Analysing Data

How to identify the alleles present in different DNA samples using PCR and agarose gel electrophoresis

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Student comment on LabSims

Quote from George-Williams et al. (2022)

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Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Genetics

Purchase option

Bioscience LabSim Library

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