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SNP Detection with CAPS

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Learn about how Cleaved Amplified Polymorphic Sequences (CAPS) can be used to determine between sequences with single nucleotide polymorphisms (SNPs).

Learning Outcomes

  • Define SNPs and CAPS
  • Predict PCR products of alleles with SNPs
  • Predict digest patterns of alleles with SNPs
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Concepts Principles and Theories

  • What is meant by a Single Nucleotide Polymorphism (SNP)
  • Why the CAPS method relies on the SNP falling within a restriction enzyme site 
  • How SNPs in restriction enzyme sites affects enzymatic activity
  • How an SNP in a restriction enzyme site does not affect the length of the expected PCR products
  • How an SNP in a restriction enzyme site does affect the length of the expected digest products

Interpreting and Analysing Data

  • How to identify the alleles present in different DNA samples using PCR and agarose gel electrophoresis

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